Biochemistry & Metabolomics

Biography

Biography: Chibuzo Carole Nweze

Abstract

Statement of problem: Diabetes mellitus is a metabolic disorder characterized with hyperglycemia and often associated with many complications in several organs most especially in liver and kidney. The purpose of this study is to evaluate effect of Pleurotusostreatus (Oyster mushroom) in Diabete mellitus and its complications. Methodology and theoretical orientation: Experimental diabetes were induced following an overnight fast, by a single intraperitoneal (i.p.) injection of Alloxan monohydrate at dose 150 mg/kg b.wt. to rats in group II to V. Control animals  (group I ) were injected with 0.5ml of normal saline. Hyperglycemia was confirmed three days after injection by measuring the blood glucose level. The Diabetic rats were then fed with broiler feeds supplemented with dried Oyster mushroom powder at doses of 10 and 20g daily for a period of four weeks whereas the control animals were given only broiler feeds. Findings: The results show that intraperitoneal (i.p.) injection of  Alloxan significantly  (p<0.05) elevated blood glucose level accompanied by significantly  (p<0.05)  increase in serum  Aspartate aminotransferase AST, Alanine aminotransferase ALT,  Alkaline phosphatase ALP, hyperlidemia,  sodium Na+, bicarbonate ion HCO₃ , potassium K+ and with a concomitant significant  (p<0.05) decrease in serum total protein , high density lipoprotein and chloride. Conclusion: Supplementation of dried oyster mushroom powder at doses of 10 and 20gdaily for a period of four weeks resulted in a reversal of the above pathological conditions associated with diabetes. Recommendation: Therefore, the study indicated that oyster mushroom besides its hypoglycemic and hypolipidemic effects could also protect the liver and kidney against impairment due to hyperglycemia.